These latter diseases are most commonly associated with Candida species, especially amongst patients with defective immune system due to cancer treatment, HIV infection, or neutropenia, but prolonged hospitalizations and the use of catheters and other medical devices are also established as predisposing factors ( Puzniak et al., 2004 Cheng et al., 2005 Li et al., 2017). parapsilosis.įungal infections represent a global threat with nearly one billion cases per year ranging from mild superficial to invasive infections with high mortality rates ( Pfaller and Diekema, 2007 Bongomin et al., 2017). Hence, our findings demonstrate that N5L is a highly effective neutral locus for knock-in approaches in C. We concluded that GFP-expressing constructs integrated into this region provide strong, homogenous fluorescent signals while alteration of this locus affects neither the growth of the mutants in liquid or on solid media, even in the presence of different stressors, nor their basic virulence properties. As a proof of principle, we fluorescently tagged laboratory and prototroph strains and established that this locus is also suitable for reintegration purposes. parapsilosis, and introduce an integrative Gateway TM and a classical ligation based replacement plasmid to target it with. Here we report CpNEUT5L (N5L), an intergenic locus in C. To investigate the virulence properties of this pathogen several techniques have been developed for generating knock-out mutants, however, no target locus for knock-in approaches have been published so far. parapsilosis whose incidence is particularly high amongst low birth weight neonates. Besides Candida albicans, other members of the genus have gained relevance in the last two decades, including C. Invasive fungal infections caused by Candida species affect approximately 700,000 people worldwide resulting in 300,000 deaths annually.
2MTA-SZTE Lendület Mycobiome Research Group, University of Szeged, Szeged, Hungary.1Department of Microbiology, University of Szeged, Szeged, Hungary.Tibor Nemeth 1, Csaba Papp 1, Csaba Vagvolgyi 1, Tanmoy Chakraborty 1 and Attila Gacser 1,2*
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